การวิเคราะห์เชิงคุณภาพการผลิตเอนไซม์ย่อยสลายไฟเบอร์ โดยบาซิลัสที่แยกจากมูลสุกรพื้นเมือง
Qualitative Analysis of Fibre-Degrading Enzymes Production by Bacillus Isolated from Native Swine Manures
Keywords:
บาซิลัส , เซลลูเลส , ไซลาเนส , เพคติเนส , การคัดกรองการสร้างเอนไซม์, Bacillus, cellulase, xylanase, pectinase, enzyme production screeningAbstract
เอนไซม์ย่อยสลายเส้นใยมีบทบาทสำคัญในการย่อยเส้นใยไฟเบอร์พอลิแซ็กคาไรด์ที่ไม่ใช่แป้ง และพอลิแซ็กคาไรด์ ที่มีโครงสร้างอื่น ๆ โดยเฉพาะเส้นใยจากพืชที่เป็นส่วนประกอบในอาหารสัตว์ มีรายงานว่าจุลินทรีย์ในลำไส้ของสัตว์สามารถผลิตเอนไซม์เหล่านี้ได้ การศึกษานี้จึงทำการคัดแยกและคัดกรองเชื้อ Bacillusspp. ที่ผลิตเอนไซม์ย่อยสลายเส้นใย ได้แก่ เซลลูเลส ไซลาเนส และเพคติเนส จากมูลสุกรพื้นเมืองที่มีสุขภาพดี จำนวน 40 ตัวอย่าง พบแบคทีเรียที่คาดว่าเป็น Bacillusspp. จำนวน 103 ไอโซเลท และจากผลการคัดกรองความสามารถในการผลิตเอนไซม์เชิงคุณภาพโดยวิธี agar plate assay พบเชื้อ Bacillus spp. จำนวน 8 สายพันธุ์ได้ แก่ Bacillus albus NA11.3 Bacillus cereus NG3.6 Bacillus cereus NM1.1 Bacillus amyloliquefaciensNL1.2 Bacillus amyloliquefaciensNL1.3 BacillussubtilisNM1.5 BacillussubtilisNM1.7 และ Bacillus subtilisNM2.2 สามารถผลิตเอนไซม์เซลลูเลส ไซลาเนส และเพคติเนสได้ระดับสูงสุด ไอโซเลทที่แยกได้ใหม่เหล่านี้มีความเหมาะสมสำหรับการศึกษาเพิ่มเติมเพื่อประเมินระดับกิจกรรมของเอนไซม์ต่อไป Fibre-degrading enzymes play an important role in fibre digestion, non-starch polysaccharides, and other structural polysaccharides in animal feed, especially in plant-based diets. It was reported that the microflora in an animal's gut could produce these enzymes. This study isolated and screenedBacillusspp. produces fibre-degrading enzymes (cellulase, xylanase, and pectinase) from native swine manures. One hundred and three isolates of the presumptive Bacillusspp. were isolated from 40faecal samples of healthy native pigs, and their qualitative ability to produce fibre-degrading enzymes was screened by agar plate assay. Eight isolates of Bacillusspp., namely, Bacillusalbus NA11.3, BacilluscereusNG3.6, Bacillus cereusNM1.1, Bacillus amyloliquefaciensNL1.2, Bacillus amyloliquefaciensNL1.3, Bacillus subtilisNM1.5, Bacillus subtilisNM1.7, andBacillus subtilisNM2.2, are the isolates that could produce cellulase, xylanase, and pectinase at the highest level. These newly isolated strains are the most suitable microorganisms for further studies to assess their quantitative enzyme production.References
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